In vitro Carrot (Daucus carota L.) Regeneration: A Study on the Use of 2,4-D and Activated Charcoal

Abstract

Regeneration can be provided with in vitro techniques in carrot (Daucus carota L.), but the response to regeneration depends on a number of factors, such as genotype, explant type, growth media, plant growth regulators and their concentrations. In the present study, Nanco and Maestro seeds were germinated, then three different types of explants (cotyledon, hypocotyl, true leaf) cultured in Murashige & Skoog (MS) media, supplemented with activated charcoal (1,0 g/l) and various doses of 2,4-Dichlorophenoxyacetic acid (2,4-D). Obtained results showed that Nanco cultivar had responded much better to in vitro regeneration than Maestro. While explant types were evaluated, it was recorded that
hypocotyls and true leaves were more responsive than cotyledons. In terms of media, MS + 2,0 mg/l 2,4-D + 1,0 g/l activated charcoal was found to be the most successful.