DIFFERENTIAL COMPETENCE FOR IN VITRO RAPID AND RELIABLE REGENERATION OF POTATO (SOLANUM TUBEROSUM L.) CULTIVARS

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Authors

  • Hussein Abdullah Ahmed AHMED Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences
  • Cansu Telci Kahramanoğulları Department of Field Crops, Agriculture Faculty, Ankara University, Ankara, Türkiye
  • Fatih Sancar Özcan Department of Field Crops, Agriculture Faculty, Ankara University, Ankara, Türkiye
  • Cennet Yaman Department of Field Crops, Agriculture Faculty, Bozok University, Yozgat, Türkiye
  • Surendra Barpete

Keywords:

Apical-node, acclimatization, microtuber, shoot regeneration, substrates

Abstract

The present study tested in-vitro growth and performance of 14 exotic and local potatoes (Solanum tuberosum L.) cultivars under artificial long-day conditions. The study carried out in four steps; 1) effect of medium (ten nutrient medium) on in vitro plant growth, 2) callus induction and shoot proliferation from internode and leaf disc explant, 3) selection of nodal explants (apical, 1st and 2nd nodes) and position, 4) acclimatization and ex-vitro plant growth of regenerated explants. The nutrient growth medium were statistically significant difference (P< 0.05) on shoot length, number of leaf/shoot and tuber formation. The M7 (MS salts + B5 vitamins) medium was most suitable and efficient for in vitro regeneration. Internode explants of 14 potatoes cultivars demonstrated significant differences (P < 0.05) among them and showed superiority for calli  induction compared to leaf disc explants. The redifferentiated from internode derived calli to adventitious shoot had positive responces on number of shoots (4.7) per plant of potato cv. Tokat-6/24 when subcultured on M7 medium containing 4 mg/l gibberellic acid, 0.5 mg/l 6-benzylaminopurine, 0.2 mg/l naphthalene acetic acid. The maximum (91.7%) acclimatization and survival rate of in vitro regenerated plantlets noted on peat moss: perlite: sand (1:1:1) when compared to other three potting mixture. The in vitro regenerated potato plants were vigorous, and produced healthy tubers in pots as well as field conditions. However, this protocol can be use for development of genetic engineer and large-scale production of healthy & disease-free potato seed tubers.

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Published

2023-01-01

How to Cite

Hussein Abdullah Ahmed AHMED, Telci Kahramanoğulları, C. ., Özcan, F. S. ., Yaman, C. ., & Surendra Barpete. (2023). DIFFERENTIAL COMPETENCE FOR IN VITRO RAPID AND RELIABLE REGENERATION OF POTATO (SOLANUM TUBEROSUM L.) CULTIVARS. International Journal of Agricultural and Natural Sciences, 15(3), 282–296. Retrieved from https://ijans.org/index.php/ijans/article/view/616

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Section

Research Articles