THE PROTECTIVE EFFECTS OF DILL (ANETHUM GRAVEOLENS L.) IN PARACETAMOL-INDUCED ACUTE TOXICATION IN MICE

Abstract

The purpose of this study is to determine whether the protective effect of the AG (Anethum graveolens) extract against experimental liver acute toxication formed by PT in mice. For this purpose, 2 months old, 20-25 g weight Balb-c male mice of 50 divided into 5 groups which each contain 10. Applications were made by oral gavage. Group I is designated as the control group and isotonic NaCl given for 7 days, Group II of animals 400 mg/kg a single dose PT applicated orally, Group III is PT+AG1 (100 mg/kg AG given for 7 days and 1 hours after last extract application 400 mg/kg PT given, Group IV is PT+AG2 (200 mg/kg AG+PT 400 mg/kg), Group V. AG2 (200 mg/kg AG were given orally for 7 days. About 5 hours after these applications the blood was taken from heart under ketamin and xylazine anesthesia; liver and kidney tissue were taken after cervical dislocation. Assessments were performed serum cytokine levels (interleukin-1ß, interleukin-6, interleukin-10 and tumor necrosis factor-α), some biochemical enzymes (ALT, AST), lipid peroxidation levels in tissues (MDA, SOD, GSH) with liver and kidney histopathological examinations. In our study, it is determined that AST, ALT, cytokine and tissue MDA levels increased, SOD and GSH levels decreased in PT group. On the other hand, when we compare the groups received two different doses (100 and 200 mg/kg) of extract with only PT applied group, AG application significantly decreased IL-1β, IL-10, IL-6, TNF-α, ALT and AST levels in serum. It is determined that MDA level decreased, and GSH and SOD levels increased depending on dose. Histopathological findings are consistent with biochemical findings. As a result, AG applications can prevent organ damage via affected cytokine answer and oxidative stress in PT-induced acute liver and kidney toxications in mice.