Volatile components of Stachys cretica subsp. anatolica Rech. F., Ann. in Koçtepe province of Isparta, Turkey

Abstract

Turkey is an important gene center in terms of the Lamiaceae family plants, which take the first place among the plant species traded on the world market and have an important role in alternative medicine applications. Stachys is one of the important genus of Lamiaceae and represented by approximately 200 taxa. In this study which was conducted in 2017 and 2018, above ground parts of Stachys cretica subsp. anatolica in flowering period of plant were collected from Koçtepe province of Isparta. Volatile components were determined by gas chromatography mass
spectroscopy (GC-MS) after solid phase micro extraction (SPME). Collected samples were placed in paper packages and transported to the laboratory on the same day and dried at room temperature (25°C), after they were subjected to solid phase microextraction (SPME). 2 g of samples were placed into a 10 mL vial and was heated to 60°C for 30 minutes. SPME apparatus for adsorbing volatiles vials passed into the upper cavity and then rested Rx-5Sil MS capillary column (30 m x 0.25 mm i.d., 0.25 μm film thickness) used in Shimadzu 2010 Plus GC-MS and injected directly into the device. That was connected to mass selector detector which is operated in hand mode (70 eV). Helium with a flow rate of 1.61 mL per minute was used as carrier gas. Injection and detection temperatures were set at 250°C for desorption (5 min) of the adsorbed volatile compounds for analysis. As result, 58 volatile components were determined and (E)-2-hexenal (12.58%), germacrene-D (34.56%) and also β-caryophyllene (21.04%) were found as main components. Stachys species are becoming increasingly important due to the aromatic compounds and essential oils they contain, as well as being the plants that are used by the public in the treatment of diseases and which are medicinal properties.