PURPLE CARROT (Daucus carota L.) GENETIC DIVERSITY OF CENTRAL ANATOLIA REVEALED BY AFLP AND ISSR

purple carrot

Abstract

The study was conducted to reveal the genetic diversity of purple carrot in Central Anatolia using AFLP (Amplified Fragment Length Polymorphism) and ISSR (Inter Simple Sequence Repeat) methods. It was aimed to estimate the level of genetic diversity and population structure among purple carrot genotypes. In total, 23 local purple carrot genotypes were collected from Hatay and Konya regions in Turkey. PCR amplification data from 6 AFLP primer combinations generated a total of 148 DNA fragments scored, 138 of which were polymorphic. Also, 12 ISSR primers utilized produced 65 fragments, 50 of which were polymorphic. Molecular data were analysed with the unweighted pair-group method arithmetic average (UPGMA) and principle coordinate analysis (PCoA). The UPGMA analysis demonstrated that the genotypes had a similarity range from 0.54 to 0.94. According to dendogram, purple carrot genotypes generally have two main branches. Structure analysis of the population using DNA markers resulted in 4 distinct subpopulations (K = 4), two of which were represented by single genotypes while the majority of the genotypes were accumulated within a specific subgroup.