ABTS ASSESSMENT AND CHEMICAL PROFILING OF Syzygium aromaticum and Cananga odorata ESSENTIAL OILS FOR ANTIOXIDANT POTENTIAL IN EAST KALIMANTAN

Abstract

This present study was to explore the antioxidant activity and total phenolic compound of two essential oils from East Kalimantan. Essential oils used in this research are Syzygium aromaticum leaves and Cananga odorata flowers. Chemical profiling of S. aromaticum leaves by GC-MS showed that eugenol (71.97%) and trans (Beta)-Caryophyllene (23.48%) are the major components. Whereas in C. odorata flowers, the major constituents are trans-caryophyllene (26.91%), Germacrene (18.15%), and alpha-bergamotene (12.41%). The Folin-Ciocalteu technique was used to determine the plant's total phenolic content. The results of a total phenolic compound of essential oil from S. aromaticum leaves and C. odorata flowers showed that the phenolic compound of gallic acid equivalents (GAE)/mg 115.20 mg GAE/g and 9.67 mg GAE/g, respectively. The free radical ABTS was utilized to investigate antioxidant activity with IC₅₀  values of S. aromaticum leaves and C. odorata flowers sequentially at 9.45 µg/mL and 21.89 µg/mL, while the ascorbic acid was 5.05 µg/mL. The finding suggests that an antioxidant test using ABTS is suitable for essential oils and S. aromaticum leaves has the potency for natural antioxidants.