Antifungal Activity Of Leaf Extract Of Ceratonia Siliqua L. Plant Against Plant Pathogenic Fungi

Abstract

Antifungal substances obtained from naturally grown plants are among the preferred products because they have a sensitive effect on the environment and on humans. In this study, the antifungal activity of the methanol extract acquired from leaves of Ceratonia siliqua plant collected from Demre district of Antalya province was determined. Activity studies have been carried out pathogens of Fusarium oxysproum f.sp. cucumerium (FOC) and Monillia fructigena plants which cause disease in cucumber and apple plants. Doses of 0.1, 0.5, 1, 2, 5 mg/ml of extract were applied against plant pathogens. Activity studies of test pathogens were carried out using the agar plate method. Mycelial growth, Mycelial growth inhibition, and Lethal dose values (LD50-90) were determined in the experiments for fungi against extracts. M. fructigena is more susceptible to the FOC pathogen. MGI ratios of 20% at 0.1 mg/ml and 92% at 5 mg/ml against M. fuctictigena were observed. Similarly, these ratios were found at 0.1 mg and 0.5 mg/ml at FOC as 11% and %79 respectively. Lethal dose rates were calculated for LD50 = 0.74 mg/ml and LD90 = 7.082 for M. fructigena and LD50 = 1.41 mg/ml and LD90 = 19.83 for FOC. According to these results, it has been determined that the extract of the C. siliqua has a high level of biological activity against the tested pathogens. Effective results of bio-antifungal substances obtained from the nature are also observed in this study.