ROLE OF DIFFERENT PARAMETERS IN THE FORMATION OF EMBRYO-LIKE STRUCTURE AND CALLUS THROUGH OVARY CULTURE IN CUCUMBER (Cucumis sativus L.)

Abstract

An instrumental aspect of modern plant breeding revolves around the production of haploid plants and the subsequent generation of double haploid (DH) plants. This innovation accelerates the process of achieving homozygosity through self-pollination, significantly truncating the timeline. In the present study, flowers of two cucumber varieties ('Kros' and 'Silah') were collected at three different stages: 12 hours before anthesis, at the time of anthesis, and 12 hours after anthesis following the sterilisation and culturing of ovaries in different induction mediums having Thidizuron (TDZ), 2,4-Dichlorophenoxyacetic acid (2,4-D) and Kinetin (Kn) were used as plant growth regulators (PGRs). Ovaries were kept at 25°C for four weeks or at 35°C and 4°C for three days and then at 25°C for the remaining days for callus induction. Callus was transferred to a maturation medium having Naphthaleneacetic acid (NAA) and benzylaminopurine (BAP) as PGRs for the development of embryo-like structure (ELS). It was concluded that TDZ and the floral stage of 12 hours before anthesis showed the best results in inducing callus and ELS through ovary culture.