DETERMINATION OF EXPRESSION LEVELS OF GENES RELATED TO ANTHOCYANIN SYNTHESIS IN SOME POMEGRANATE GENOTYPES
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qRT-PCR, gene, RNA, CHS, CHIAbstract
Pomegranate is a perennial deciduous fruit-bearing shrub with significant commercial value and a substantial place in cultural life. Pomegranate seeds are rich in phenolics and have various positive impacts on human health. Therefore, there is an ever-increasing interest in pomegranate and also in the consumption of fruits species with high anthocyanin contents. In the present study, expression levels of 4 different anthocyanin synthesis-related genes (CHS, CHI, F3H, PAL) in peel, seed and leaf tissues of 3 different pomegranate genotypes (33N53, İzmir-1479 and 19/147) were investigated with the use of qRT-PCR technique. Present analyses revealed that anthocyanin synthesis-related gene expression profiles were low in white genotype, medium in pink genotype and high in red genotype. In general, high expression levels were observed in CHS and CHI genes.
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